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mouse monoclonal perk  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse monoclonal perk
    Mouse Monoclonal Perk, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 5877 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+monoclonal+perk/p-ERK+Antibody/pmc12951383-53-25-30
    Average 96 stars, based on 5877 article reviews
    mouse monoclonal perk - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Immunohistochemical Expression of IRE1 and PERK in Breast Cancer: Associations With Clinicopathological Characteristics and Survival Outcomes
    Article Snippet: IHC staining was performed using the automated BOND-MAX system (Leica Biosystems, Nussloch, Germany). .. After deparaffinization and antigen retrieval (EDTA, 98 ̊C, 20 min), peroxidase blocking was applied for 10 min. Tissue sections were incubated with primary antibodies rabbit polyclonal IRE-1 (E-AB-93217, Elabscience, Houston, TX, USA) and mouse monoclonal PERK (B-5: sc-377400, Santa Cruz, Dallas, TX, USA), both at 1:50 dilution, followed by polymer incubation (10 min) and DAB chromogen application (10 min). .. Sections were counterstained with Mayer’s hematoxylin, dehydrated, cleared in xylene, and examined under a Nikon Eclipse E200 microscope.

    Incubation:

    Article Title: Immunohistochemical Expression of IRE1 and PERK in Breast Cancer: Associations With Clinicopathological Characteristics and Survival Outcomes
    Article Snippet: IHC staining was performed using the automated BOND-MAX system (Leica Biosystems, Nussloch, Germany). .. After deparaffinization and antigen retrieval (EDTA, 98 ̊C, 20 min), peroxidase blocking was applied for 10 min. Tissue sections were incubated with primary antibodies rabbit polyclonal IRE-1 (E-AB-93217, Elabscience, Houston, TX, USA) and mouse monoclonal PERK (B-5: sc-377400, Santa Cruz, Dallas, TX, USA), both at 1:50 dilution, followed by polymer incubation (10 min) and DAB chromogen application (10 min). .. Sections were counterstained with Mayer’s hematoxylin, dehydrated, cleared in xylene, and examined under a Nikon Eclipse E200 microscope.

    Article Title: Immunohistochemical Expression of Endoplasmic Reticulum Stress Markers and their Association With Clinicopathological Characteristics and Survival Outcomes in Endometrial Cancer
    Article Snippet: .. For the detection of IRE1 and PERK expression, tissue sections were incubated with specific primary antibodies: rabbit polyclonal IRE1 (E-AB-93217; Elabscience, Houston, TX, USA) and mouse monoclonal PERK (B-5: sc-377400; Santa Cruz Biotechnology, Dallas, TX, USA), both at a dilution of 1:50. .. Following primary antibody incubation, a polymer-based detection system was applied for 10 min, followed by visualization using BOND Polymer Refine Detection DAB Chromogen (Cat. No. DS9800; Leica Biosystems) for another 10 min. Counterstaining was performed using Mayer’s hematoxylin, after which the slides were sequentially dehydrated in graded alcohol solutions and cleared in xylene.

    Article Title: Immunohistochemical Analysis of IRE1 and PERK Expression in Extravillous Trophoblast Cells of Placenta Accreta Spectrum and Associations With Clinicopathological Parameters
    Article Snippet: .. The sections were incubated with primary antibodies: rabbit polyclonal IRE1 (E AB 93217; Elabscience, Houston, TX, USA) and mouse monoclonal PERK (B 5; sc 377400; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), both applied at a dilution of 1:50. ..

    Article Title: Immunohistochemical Analysis of IRE1 and PERK Expression in Extravillous Trophoblast Cells of Placenta Accreta Spectrum and Associations With Clinicopathological Parameters.
    Article Snippet: .. The sections were incubated with primary antibodies: rabbit polyclonal IRE1 (E AB 93217; Elabscience, Houston, TX, USA) and mouse monoclonal PERK (B 5; sc 377400; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), both applied at a dilution of 1:50. ..

    Polymer:

    Article Title: Immunohistochemical Expression of IRE1 and PERK in Breast Cancer: Associations With Clinicopathological Characteristics and Survival Outcomes
    Article Snippet: IHC staining was performed using the automated BOND-MAX system (Leica Biosystems, Nussloch, Germany). .. After deparaffinization and antigen retrieval (EDTA, 98 ̊C, 20 min), peroxidase blocking was applied for 10 min. Tissue sections were incubated with primary antibodies rabbit polyclonal IRE-1 (E-AB-93217, Elabscience, Houston, TX, USA) and mouse monoclonal PERK (B-5: sc-377400, Santa Cruz, Dallas, TX, USA), both at 1:50 dilution, followed by polymer incubation (10 min) and DAB chromogen application (10 min). .. Sections were counterstained with Mayer’s hematoxylin, dehydrated, cleared in xylene, and examined under a Nikon Eclipse E200 microscope.

    Expressing:

    Article Title: Immunohistochemical Expression of Endoplasmic Reticulum Stress Markers and their Association With Clinicopathological Characteristics and Survival Outcomes in Endometrial Cancer
    Article Snippet: .. For the detection of IRE1 and PERK expression, tissue sections were incubated with specific primary antibodies: rabbit polyclonal IRE1 (E-AB-93217; Elabscience, Houston, TX, USA) and mouse monoclonal PERK (B-5: sc-377400; Santa Cruz Biotechnology, Dallas, TX, USA), both at a dilution of 1:50. .. Following primary antibody incubation, a polymer-based detection system was applied for 10 min, followed by visualization using BOND Polymer Refine Detection DAB Chromogen (Cat. No. DS9800; Leica Biosystems) for another 10 min. Counterstaining was performed using Mayer’s hematoxylin, after which the slides were sequentially dehydrated in graded alcohol solutions and cleared in xylene.

    Western Blot:

    Article Title: In vitro selenium supplementation suppresses key mediators involved in myometrial activation and rupture of fetal membranes.
    Article Snippet: .. Western blotting was performed as previously described.36 Membranes were probed with 0.2 mg ml 1 mouse monoclonal pERK (sc-7383; Santa Cruz Biotechnology) and 0.2 mg ml 1 rabbit polyclonal ERK (sc-93, Santa Cruz Biotechnology), and viewed and analysed using the ChemiDoc MP system (Bio-Rad Laboratories; Gladesville, NSW, Australia). .. Semi-quantitative analysis of the relative density of the bands in western blots was performed using Quantity One 4.2.1 image analysis software (Bio-Rad Laboratories, Hercules, CA, USA).



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    pCP312R promotes the phosphorylation of <t>PERK</t> and eIF2 α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), <t>anti-PERK,</t> or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis using anti-Flag, anti-p-eIF2 α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.
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    Image Search Results


    pCP312R promotes the phosphorylation of PERK and eIF2 α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis using anti-Flag, anti-p-eIF2 α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.

    Journal: Veterinary Research

    Article Title: The CP312R protein of African swine fever virus inhibits host protein translation via the BiP/PERK/eIF2 α pathway

    doi: 10.1186/s13567-025-01688-5

    Figure Lengend Snippet: pCP312R promotes the phosphorylation of PERK and eIF2 α . A pCP312R promotes the phosphorylation of PERK in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 24 h post-transfection (hpt), the cells were collected for western blotting analysis using anti-Flag, anti-p-PERK (Thr982), anti-PERK, or anti- β -actin antibodies. B pCP312R promotes eIF2 α phosphorylation in a dose-dependent manner. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 0.5, 1, 1.5, or 2 μg). At 12 hpt, the cells were incubated with 300 nM thapsigargin (Tg) for 8 h, and then the cells were collected for western blotting analysis using anti-Flag, anti-p-eIF2 α , anti-eIF2 α , or anti- β -actin antibodies. C Inhibition of the cellular protein synthesis by pCP312R is attenuated by ISRIB. HEK293T cells were transfected with an increasing amount of pCA-CP312R-Flag (0, 1, 1.5, 2, or 2.5 μg). At 12 hpt, the cells were treated with ISRIB (0.5 μM) for another 12 h. The cells were pulsed with 3 μM puromycin for 30 min, followed by western blotting analysis using anti-Flag, anti-puromycin, or anti- β -actin antibodies.

    Article Snippet: Mouse anti-p72 monoclonal antibody (MAb) (catalog no. Anti PPA 1BC11, INGENASA), mouse anti-puromycin MAb (catalog no. MABE343, Sigma-Aldrich), rabbit anti- β -actin PAbs (catalog no. AC026, ABclonal), mouse anti-Flag MAb (catalog no. M8823, Sigma-Aldrich), mouse anti-GST MAb (catalog no. K200006M, Solarbio), rabbit anti-Myc PAbs (catalog no. YN5506, ImmunoWay), rabbit anti-KDEL PAbs (catalog no. ab214714, Abcam), rabbit anti-BiP PAbs (catalog no. A0241, ABclonal), mouse anti-PERK MAb (catalog no. 3192S, CST), rabbit anti-p-PERK PAbs (catalog no. BS66100, Bioworld), rabbit anti-p-eIF2 α PAbs (catalog no. 3398S, CST), and rabbit anti-eIF2 α PAbs (catalog no. 9722S, CST) are commercially available.

    Techniques: Phospho-proteomics, Transfection, Western Blot, Incubation, Inhibition

    A schematic model illustrating the inhibition of cellular protein synthesis by the ASFV pCP312R . In addition to interaction with RPS27A , pCP312R downregulates the expression of heavy-chain-binding protein (BiP) and promotes the phosphorylation of the PKR-like endoplasmic reticulum kinase (PERK) and the eukaryotic initiation factor 2 α (eIF2 α ), thereby inhibiting cellular protein synthesis.

    Journal: Veterinary Research

    Article Title: The CP312R protein of African swine fever virus inhibits host protein translation via the BiP/PERK/eIF2 α pathway

    doi: 10.1186/s13567-025-01688-5

    Figure Lengend Snippet: A schematic model illustrating the inhibition of cellular protein synthesis by the ASFV pCP312R . In addition to interaction with RPS27A , pCP312R downregulates the expression of heavy-chain-binding protein (BiP) and promotes the phosphorylation of the PKR-like endoplasmic reticulum kinase (PERK) and the eukaryotic initiation factor 2 α (eIF2 α ), thereby inhibiting cellular protein synthesis.

    Article Snippet: Mouse anti-p72 monoclonal antibody (MAb) (catalog no. Anti PPA 1BC11, INGENASA), mouse anti-puromycin MAb (catalog no. MABE343, Sigma-Aldrich), rabbit anti- β -actin PAbs (catalog no. AC026, ABclonal), mouse anti-Flag MAb (catalog no. M8823, Sigma-Aldrich), mouse anti-GST MAb (catalog no. K200006M, Solarbio), rabbit anti-Myc PAbs (catalog no. YN5506, ImmunoWay), rabbit anti-KDEL PAbs (catalog no. ab214714, Abcam), rabbit anti-BiP PAbs (catalog no. A0241, ABclonal), mouse anti-PERK MAb (catalog no. 3192S, CST), rabbit anti-p-PERK PAbs (catalog no. BS66100, Bioworld), rabbit anti-p-eIF2 α PAbs (catalog no. 3398S, CST), and rabbit anti-eIF2 α PAbs (catalog no. 9722S, CST) are commercially available.

    Techniques: Inhibition, Expressing, Binding Assay, Phospho-proteomics